Cytc antibody
WebCytochrome c (Cytc) 1 is a cellular life and death decision molecule that regulates cellular energy supply and apoptosis through tissue specific post-translational modifications. Cytc is an electron carrier in the mitochondrial electron transport chain (ETC) and thus central for aerobic energy production. Webcytochrome c Antibody (A-8) is an IgG 2b κ mouse monoclonal cytochrome c antibody (also designated CYCS antibody) that detects the cytochrome c protein of mouse, rat and human origin by WB, IP, IF, …
Cytc antibody
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WebCytochrome c has been used to detect peroxide production in biological systems. As superoxide is produced, the number of oxidised cytochrome c 3+ increases, and reduced cytochrome c 2+ decreases. [36] However, … WebOur antibody specificities are confirmed for multiple applications, and all our flow cytometry reagents are titrated on the relevant positive and/or negative cells. Find more information …
WebTHE CytoCx DIFFERENCE. As a medical brand, CytoCx understands that the only way to guarantee quality is to control every step in the production. That is why we cultivate our … WebNov 11, 2024 · Cytochrome c (Cytc), an endogenous heme-based protein, holds tremendous potential to treat gliomas because of its innate capacity to trigger apoptosis. To this end, a hybrid cytochrome c-chlorotoxin (Cytc-CTX) protein was biosynthesized to enable cellular uptake of the cell impenetrable Cytc using CTX transporters.
WebCytochrome c belongs to class I of the c-type cytochrome family and contains a characteristic CXXCH (cysteine-any-any-cysteine-histidine) amino acid motif that binds heme. This motif is located towards the N … WebApr 1, 2024 · The cytosolic fraction of cyt c was analyzed by western blot, as previously described. Briefly, cells were harvested, washed twice with ice-cold PBS and incubated in ice-cold Tris-sucrose buffer (0.35 mM of sucrose, 10 mM of Tris-HCl at pH 7.5, 1 mM of EDTA, 0.5 mM of dithiothreitol, and 0.1 mM of phenylmethylsulfonyl fluoride).
WebIn parallel to these early studies on cytotoxic antibody effector functions, it was noted that antibodies in combination with their respective antigen resulted in binding of these …
WebCytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. Upon apoptotic stimulation, … shsc policies and proceduresWebSep 21, 2009 · Slides were further incubated with monoclonal anti-cyto-chrome c (CytC) antibody (BD Biosciences; 1:200 in PBST containing 5 mg/ml BSA; 2 h, room temperature) followed by Alexa Fluor 488-conjugated anti-mouse IgG (Invitrogen; 1:200 in PBST with 5 mg/ml BSA; 1 h, room temperature). Slides were mounted and images were acquired … theory research paperWebJan 24, 2012 · Anti KdgM and anti CytC antibodies were detected with anti IgG labelled with Alexa fluor 488 (green labelling) and DNA was stained with DAPI (blue labelling). … theory resident portalWebAug 3, 2013 · Furthermore SUG increase CytC, AIF, Smac/Diablo and CASP-3 protein expression in cells in culture. ... Barcelona, Spain). Monoclonal anti-glial fibrillary acidic protein (anti-GFAP) antibody (1:500) was from Sigma-Aldrich Biotech (Madrid, Spain). Monoclonal anti-MAP-2 protein (anti-MAP-2) antibody (1:500) and monoclonal anti … sh scoundrel\u0027sWebAqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol. This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only. Store at -20°C, for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. shs coursesWebCytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. shsc pharmacyWebTested in Flow Cytometry (Flow) applications. This antibody reacts with Human, Non-human primate samples. Supplied as 100 Tests purified antibody (5 µL/Test). ... and curves of the dynamic concentration changes of metabolites (FAD, Cytaa3-R, and Cytc-R) in the region of the hind limb underwent RLIPoC in vivo, which was monitored using a ... shs corvallis