Cdk1 in-cell western
WebFeb 16, 2016 · Mechanistically, CDK1 inhibition caused a reduction in the S-phase fraction of KRAS mutant cells, an effect also characterised by modulation of Rb, a master control of the G 1 /S checkpoint. Taken together, these observations suggest that the KRAS/CDK1 interaction is a robust synthetic lethal effect worthy of further investigation. Figures WebMay 20, 2024 · GCP Targets PLK1 and CDK1 to Inhibit Their Expression in HeLa Cells. CDK1 and PLK1 are critical cellular regulators, which play a vital role in cell differentiation, proliferation, and autophagy. Therefore, we examined the effect of 1,500 nM GCP on the protein expression levels of CDK1 and PLK1 at 24, 48, and 72 h in HeLa cells by …
Cdk1 in-cell western
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WebApr 25, 2013 · Figure 2. CDK1 expression in human hepatic cancer tissues and cell lines. (A) The expression levels of CDK1 were determined in 48 pairs of human hepatic cancer tissues (T) and their corresponding … WebFormation of an active nuclear cyclin B1-CDK1 complex is a highly intricate procedure requiring many different levels of regulation. Each of these regulatory steps represents a potential target for controlling cell proliferation. Accumulation of threshold levels of cyclin B1 protein at the G2/M transition requires the cooperation of various ...
WebCDK1 (CDC2, P34CDC2) is a member of the Ser/Thr protein kinase family. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S … WebThe single-cell analysis also revealed a higher degree of variability within the population of CDK1 KD cells, which might reflect cell-to-cell differences in KD efficiency despite the …
WebAug 14, 2009 · Cdk2 and cdk1 are individually dispensable for cell-cycle progression in cancer cell lines because they are able to compensate for one another. However, shRNA-mediated depletion of cdk1 alone or small molecule cdk1 inhibition abrogated S phase cell-cycle arrest and the phosphorylation of a subset of ATR/ATM targets after DNA damage. … WebFeb 16, 2016 · CDK1 phosphorylation levels in KRAS mutant and WT cells as shown by Western blot analysis of total cell protein lysates from SW48 KRAS isogenic (A), non …
WebJun 27, 2024 · Cyclin dependent kinase 1 (CDK1) has been primarily identified as a key cell cycle regulator in both mitosis and meiosis. Recently, an extramitotic function of CDK1 emerged when evidence was found that CDK1 is involved in many cellular events that are essential for cell proliferation and survival. I …
WebPolyclonal Antibody for studying Chk1 (Ser345) phosphate. Cited in 358 publications. Validated for Western Blotting. Available in 3 sizes. Highly specific and rigorously validated in-house, Phospho-Chk1 (Ser345) Antibody (CST #2341) is ready to ship. laura pittman mdWebApr 25, 2015 · Furthermore, Western blot analysis was performed for CDK1 in EOC samples and cell lines to evaluate their protein levels and molecular interaction. Kaplan-Meier survival analysis showed that strong expression of CDK1 exhibited a significant correlation with poor prognosis in human EOC ( P = 0.02). laura poelmansWebFeb 4, 2024 · Mechanistically, CDK1 phosphorylates BLM and TOPBP1 and promotes the interaction of both proteins with PLK1. This is amplified by the ability of TOPBP1 to facilitate phosphorylation of BLM at sites that stimulate both BLM-PLK1 and BLM-TOPBP1 binding, creating a positive feedback loop that drives rapid BLM phosphorylation at the G 2 -M … laura pittman npWebAug 14, 2013 · the natural Cdk1 substrate, PP1C a . By combining this substrate in a western blot method using fluo- rochrome based antibodies and phospho-imager analysis, we measured the K m of ATP binding to... laura pohjola ouluWebAug 16, 2007 · Unicellular organisms such as yeasts require a single cyclin-dependent kinase, Cdk1, to drive cell division 1. In contrast, mammalian cells are thought to require … laura pohlmannWebNov 6, 2014 · Cyclin-dependant kinase 1 (CDK1) is a key player in cell cycle regulation and particularly mitosis; however, its role has not been studied previously in hESC and … laura pittman niuWebFeb 21, 2012 · The activities of cyclin-dependent kinases (Cdks) control all aspects of cell division, including entry into the cell cycle from quiescence, the G1/S phase transition, DNA replication in S phase, nuclear … laura poinsotte